Charge-State Distribution of Aerosolized Nanoparticles

In single-particle imaging experiments, beams of individual nanoparticles are exposed to intense pulses X-rays from free-electron lasers record diffraction patterns single, isolated molecules. The reconstruction for structure determination relies on signals many identical particles. Therefore, well-defined-sample delivery conditions desired in order achieve sample uniformity, including avoidance charge polydispersity. We have observed charging 220 nm polystyrene particles an aerosol beam created by a gas-dynamic virtual nozzle focusing technique, without intentional the nanoparticles. Here, we present deflection method detecting and characterizing states aerosolized Our analysis charge-state distribution using optical light-sheet localization microscopy quantitative particle trajectory simulations is consistent with previous descriptions skewed probabilities triboelectrically charged